Kinetic studies on the reaction between native globin and haem derivatives.
نویسندگان
چکیده
منابع مشابه
Changes in Side Chain Reactivity Accompanying the Binding of Heme to Sperm Whale Apomyoglobin.
Considerable attention has been devoted recently to the study of conformational changes occurring during enzyme-substrate interaction (1, 2). Presumably the nature of the interaction between apoproteins and their prosthetic groups is essentially similar to that between enzymes and substrates, with the exception that no catalytic step occurs subsequent to the initial binding. Studies of the phys...
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It was possible to account for the time course of the reactions of both carboxyheme and monocyanide heme with globin by use of suitable values for k3 and the ratio kr/kz, the two latter constants being too great to measure directly by the stopped flow method. It has been known for more than 30 years that mesoand deuterohemes can combine with globin to form compounds able to react reversibly wit...
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Seven components of the tetrameric haemoglobin (Hbu) from Urechis caupo were separated by preparative isoelectric focusing and characterized by their absorption spectra and pI values. The helix content and Soret delta epsilon values are reported for several of the components. Temperature-jump O2-binding kinetics of the major components of Hbu show biphasic behaviour, with the majority species h...
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Ligand-binding kinetics of native and reconstituted sperm-whale myoglobin were studied in relation to haem orientational disorder by rapid kinetic methods. In addition, native yellow-fin-tuna myoglobin with significant amount of haem disorder was also used. The O2 dissociation and association rates were found for the proteins with different degrees of haem disorder, and these results suggest th...
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The following factors were measured for extracellular haemoglobins of Artemia salina: a minimal molecular weight of globin chain per haem group (based on the iron and haem contents), the absorption coefficients, the absorption spectra of various derivatives and the amino acid compositions. These were compared with those of the haemoglobins of other invertebrates. Three Artemia haemoglobins (I, ...
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عنوان ژورنال:
- The Biochemical journal
دوره 77 شماره
صفحات -
تاریخ انتشار 1960